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however, the only statistically significant difference was between the LD control group and both groups switched from WD to LD, regardless of their treatment (Supplementary Fig

That is a genetic-knockdown study in mice, not a drug trial: it tells you the target is real and metabolically meaningful in an animal model, but it does not test a drug, a dose, or a route

This could theoretically affect DNA methylation, gene expression, and other processes dependent on methylation

Although fundamental 5A-1MQ and LD-mediated changes to the microbiome were identified, this work is similar to many current studies where further investigations will clarify if changes in the microbiome are causative or associative 100