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Materials and Methods Yeast cultivation and expression of the NTD The DNA sequence of the N-terminal domain of the NCR1 protein (residues 1249, UniProt: Q12200) was introduced into an expression construct based on p423-GAL1, with a C-terminal thrombin cleavage site and a deca-histidine tag for purification (64)
Heart-Shaped Lips Characteristics : Full lower lip with a tapered upper lip (resembling a heart)
T., Varambally S., Beecher C., Chinnaiyan A

In this study, the methods of methanol addition were: (1) one step: methanol was added in one step
