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Ramakrishnan B, Megharaj M, Venkateswarlu K, Sethunathan N, Naidu R (2011) Mixtures of environmental pollutants: effects on microorganisms and their activities in soils
About this article Cite this article Ouyang, C., Liu, P., Liu, Y
Briefly, 1 mL of assay mixture contains optimized concentrations of the following chemicals: 0,5 M K 2 HPO 4 (pH 7.0), 2,5 mM EDTA, 0,18 U/mL GR, 100 mM glutathione and 10 mM reduced NADPH and tissue extract (0,5 mL) was added in the spectrophotometer cuvette along with 0,1 mL of 60 mM cumene hydroperoxide, a suitable substrate for GP
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