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BV-2 cells were first seeded at a density of 1 10 5 cells/well in a 24-well plate for 24 h, pretreated with the test agents (equivalent to 18 M ASX) for 6 h, and incubated with medium containing 500 ng/mL LPS, except for the control group

These observations were further confirmed by Hoechst 33342 staining, which revealed extensive detachment of cells from the cell culture substratum and increases in apoptotic bodies of HepG2 cells incubated with H 2 O 2 and TNF for 16 h (Fig

Oxidative stress plays a significant role in Parkinsons disease, marked by the accumulation of free radicals, lipid peroxidation, and impaired mitochondrial function

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