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Following this period, the mice were randomly divided into five treatment groups ( n = 5 per group): PBS, Mn/TI@mHSA, P-Mn@mHSA, P-Mn/TI@mHSA-N and P-Mn/TI@mHSA group, with the doses equivalent for 4 mg kg 1 of Mn/TI@mHSA