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This pain usually begins in infancy and continues throughout life, necessitating preventive rather than treatment measures [2]

This means that changes in epigenetic marks caused by pesticide exposure in one generation could potentially influence the reproductive health and development of the offspring ( The potential of epigenetic modifications to mediate the effects of pesticide exposure on reproductive health highlights the complexity of environmental influences on human biology (156)

To verify protein expression, a small aliquot of the supernatant was mixed with protein loading buffer, boiled at 100 C for 3 min, and analyzed by SDS-PAGE
