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High-Quality Protein Sources Your body needs adequate amounts of the amino acids cysteine, glycine, and glutamic acid to produce glutathione

For this assay, we utilized a construct, SI N -SI TM -GFP, encoding the N-tail and transmembrane segment of the plasma membrane protein, sucrase isomaltase (SI), fused to GFP

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2.2.3 Western blot analysis Protein levels of total pyruvate dehydrogenase E1 subunit alpha (PDHA1) and phosphorylated PDHA1 at Ser 293 (pPDHA1) were analyzed in the cytosolic fraction prepared from fresh liver tissue, while representative subunits of mitochondrial oxidative phosphorylation (OXPHOS) complexes IV were determined in whole cell lysates from frozen liver tissue as previously described ( For Western blot analysis, 30 g protein per sample were mixed with loading buffer, denatured at 95 C for 5 min (PDHA1 and pPDHA1) or at 37 C for 20 min (OXPHOS complexes) and separated on a TGX Stain-Free Precast gradient gel (Bio-Rad Laboratories GmbH, Germany)