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This shift provides information about the local environments polarity, dynamics, and rigidity, making it useful for monitoring protein stability, conformational changes, and ligand binding

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non-conservative amino acid substitution refers to the amino acids substitution of an amino acid in one class with an amino acid from another class for example, substitution of an Ala, a class II residue, with a class III residue such as Asp, Asn, Glu, or Gin

In addition, two siblings also had spinocerebellar degeneration, peripheral neuropathy, myopathy and aminoaciduria [3, 8]