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Though in vitro data and in vivo animal data thus far is promising, more information and human trials are needed to evaluate the potential role of methylene blue as a treatment for acute and chronic brain injuries

When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr

Roberts, S., Evans, E