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Lentiviral particles were generated by co-transfecting Lenti-X 293 T cells (Takara, Kyoto, Japan) with the AGTR1-expressing or control plasmid and three packaging plasmids: PMDLg./pRRE pRSV-Rev (RRID: Addgene_12253), and pVSV-G (RRID: Addgene_138479)

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