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The prevalence of acne is 51% among women aged 2029 years, but it is 27% among women aged 4049 years
The reaction was triggered by the addition of the required amount of solubilized mitochondrial sample and the absorbance change was recorded at 550 nm over 2 min by enzyme-linked immunosorbent assay (ELISA) microplate absorbance reader (Biorad-I USA)

Embora no existam contraindicaes por faixa etria, recomenda-se o uso apenas por pacientes adultos
