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By using a PACE compatible splitintein pIII that functions as an AND gate to join two orthogonal positive selections, SE-PACE is able to develop proteins in the presence or lack of concurrent selection for protein action and improve the expression of a wide range of proteins, along with bacterially derived proteins (MBP), genetically modified antibodies (scFv), and eukaryotic enzymes, as well as proteins with lower stability that have developed via protracted directed evolution

doi: 10.1073/pnas.97.10.5440 [DOI] [PMC free article] [PubMed] [Google Scholar] 81

Nevertheless, some data now suggest that a daily administration of folic acid at a dose lower than 1 mg may be adequate to reverse hyperhomocysteinemia (Boushey et al, 1995

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