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Human iPSCs were adapted onto feeder-free conditions by plating on Geltrex (Thermo Fisher Scientific, Rockford, IL) coated plates, and fed daily with mTeSR medium (Stem Cell Technologies, Vancouver, Canada) at 37C 5% CO 2

Recent research has shown that SCFAs play a crucial role in modulating the structure and function of the BBB

The ability to differentially enhance GluN2C- and GluN2D-containing NMDARs over GluN2A- and GluN2B-containing receptors may reflect the relatively low basal open probability, allowing PAMs to produce greater fold potentiation of GluN2C- and GluN2D-containing NMDARs

M.GrabowskaI.KasprzyckaP.et al (2020)