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All these cells were added into 8-well confocal plate under normal or hypoxic conditions, allowing for suitable growth density (60%)

The metabolites of the gut microbiota may affect the synthesis of neurotransmitters through the tryptophan metabolism pathway and interfere with the brain's regulation of emotions (18)

For example, the reduction of pyruvate to lactate, as catalyzed by lactate dehydrogenase, is accompanied by the oxidation of NADH to NAD + , as shown in the following two half-reactions: To evaluate the driving force for the full reaction to occur, one would consider the standard reduction potential, E, associated with each of the individual half reactions

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