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It plays a crucial role in the citric acid cycle (Krebs cycle), which is essential for energy production at the cellular level

TMA-containing compound assay Trimethylamine-containing compounds were extracted from 50 l of plasma, 50 l of whole liver homogenate or from 100 l of whole brain homogenate (without the cerebellum and olfactory bulbs) by adding 10 volumes of ACN and spiking the samples with 5 l of IS mix containing 12 M d9-TMAO (CIL), 649 nM d9-TMA (CDN Isotopes), 6 M d3-L-carnitine (CIL), 329 nM d9-crotonobetaine (see Koeth et al., 2014) and 5 M d9-TML (Santa Cruz Biotechnology) at the beginning of the extraction

Carnitine is a compound that carries fatty acids into the cell's mitochondria where they are burned for energy

(More information) Moreover, elevated blood concentrations of trimethylamine N-oxide (TMAO), generated from choline, have been associated with an increased risk of cardiovascular events in some observational studies