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As cited above, the amino acid cysteine is a rate-limiting factor for GSH synthesis, and a variety of both clinical trials and in-vitro/in-vivo data suggest that supplying cysteine as NAC is an effective strategy for enhancing GSH production and intracellular cysteine.Intravenous NAC has been utilized for some time to treat acetaminophen and non-acetaminophen induced acute liver failure by restoring the concentration of GSH, and has been found to be safe and effective

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Therefore, we speculated that CsGSTs could bind to and activate the thiol group of GSH, and the S-glutathionylated xenobiotics would be sequentially processed to participate in the cold stress response