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Kruse T, Hansen JL, Dahl K, Schaffer L, Sensfuss U, Poulsen C, et al

doi:10.1007/s10517-011-1453-2

The cell lysates (1ml) were mixed 1:1 with 85% sucrose solution, layered in the bottom of the centrifuge tube (Ultra clear Beckman centrifuge tubes) and overlaid sequentially with 6ml 30% and 3.5ml 5% sucrose solution to make a non-continuous sucrose gradient

The mechanism of action of N-acetylcysteine (NAC): the emerging role of H(2)S and sulfane sulfur species
