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174 NEs are thermodynamically unstable and kinetically stable systems
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Lysate concentration was determined using Bradford assay (BSA standard) and diluted to 1 mg ml 1 and incubated with tetrazine-Cy5 (1 M, 30 minutes, 37 C) and then quenched with 5 l of 4 Laemmeli dye containing 6% -mercaptoethanol
