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Amon J, Brau T, Grimrath A, Hanssler E, Hasselt K, Holler M, Jessberger N, Ott L, Szokol J, Titgemeyer F, Burkovski A: Nitrogen control in Mycobacterium smegmatis: nitrogen-dependent expression of ammonium transport and assimilation proteins depends on the OmpR-type regulator GlnR

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hinT variants display varying specificity and sensitivity to AMPylated substrates To analyze the binding and detection limits of hinT homologs to previously characterized AMPylated proteins, we performed dot blot assays using serial dilutions of AMPylated proteins and their corresponding non-AMPylated controls