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Description
A web of circadian pacemakers
Polyhistidine-tagged proteins can be purified under native or denaturing (28M urea or guanidine-HCl) conditions
Samuelov, L
In brief, 0.5 ml of the supernatant of brain homogenate was added to exactly 4.5 ml of working reagent (1 volume from 0.8% thiobarbituric acid + 3 volume from 20% trichloroacetic acid)
