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This differentiated status of DMSO-treated HepaRG cell cultures was routinely checked by their phase-contrast microscopy analysis, demonstrating the presence of hepatocyte-like islands and the formation of bile canaliculi, as previously reported ( 39 )

Therefore, future clinical research should incorporate rigorous doseresponse studies, long-term safety evaluations, and systematic monitoring of potential drugnutrient and nutrientnutrient interactions to ensure both efficacy and safety in translational applications

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