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Mechanistically, these agents activate the Nrf2/ARE, NF-B, MAPK, PI3K/Akt/mTOR, and SIRT1/PGC-1 pathways, protect mitochondria and the bloodnerve barrier, and upregulate endogenous antioxidant enzymes

Microscopic studies were carried out using a Zeiss Axiowert 200 M microscope (Carl Zeiss, Jena, Germany) equipped with a high-resolution digital camera (Axiocam HR, HQ CCD, Carl Zeiss, Jena, Germany) and filter set 10 (exc.: 535585 nm, em.: 600655 nm) for FDA, filter set 9 (exc.: 450490 nm, em.: 515 nm) for DHE, or filter set 20HE (exc.: 546/12, em.: 607/80) for Amplex Red was used

Skin & ECM Remodeling Published cell and animal studies have measured GHK-Cu-driven increases in collagen, decorin, and glycosaminoglycan synthesis in dermal fibroblasts, supporting its use as a reference tool in skin biology and ECM research