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Kawano H, Cho K, Haruna Y, et al
( KO 32 )
[DOI] [PubMed] [Google Scholar] 331.Wallen ZD, et al
Reverse transcription was applied at 50 C for 30 min, a primary denaturation step was done at 95 C for 5 min, followed by 35 cycles of 94 C for 30 s., annealing (55 C for 40 s for bovine viral diarrhea virus)
