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To test whether the special localization of ORF3a was important for its function in autophagy, different truncated ORF3a proteins were constructed and tested for their function in autophagy (Fig

( A -R (Cat: ab92747, Abcam, Cambridge, United Kingdom), GABA B -R1 (Cat: PA5-27725, Thermo Fisher Scientific), GABA B -R2 (Cat: ab52248, Abcam, Cambridge, United Kingdom), 5-HT 1A (Cat: ab85615, Abcam, Cambridge, United Kingdom), GluA1 (Cat: ab31232, Abcam, Cambridge, United Kingdom), GluN1 (Cat: ab17345, Abcam, Cambridge, United Kingdom), Glun2A (Cat: ab203197, Abcam, Cambridge, United Kingdom), endothelial nitric oxide synthase (eNOS) (Cat: ab5589, Abcam, Cambridge, United Kingdom), and inducible nitric oxide synthase (iNOS) (Cat: ab178945, Abcam, Cambridge, United Kingdom) were added for overnight incubation