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DIA files were processed using Spectronaut with default settings, with PTM localization activated and site confidence score cutoff set to 0 (for peptide-level analysis of dilution benchmark), 0.75 (for spectral library analysis) or 0.99 (for direct DIA analysis), data filtering set to Q- value and Normalization Strategy set to Local Normalization

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But vigorous or prolonged shaking can denature the peptide through mechanical stress

[Normalizing effect of the pineal gland peptides on the daily melatonin rhythm in old monkeys and elderly people]