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Brummel K, Eerkens AL, de Bruyn M, Nijman HW

Identification of a suppressive mechanism for Hedgehog signaling through a novel interaction of Gli with 14-3-3
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At the desired time point, cells were fixed with 4% PFA for 45 min, permeabilized with 0.2% Triton X-100 (Sigma, 11332481001) diluted in phosphate-buffered saline (PBS) for 10 min and blocked with 1% bovine serum albumin (Sigma, cat