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KCl 2.7 mM, KH 2 PO 4 1.5 mM, NaCl 136 mM, Na 2 HPO 4 2 H 2 O 6.5 mM, pH 7.4) containing 3% bovine serum albumin (BSA-PBS) for 1 h at room temperature and were then incubated with a rabbit anti-claudin 5 primary antibody diluted in 3% BSA-PBS (1:300) overnight at 4 C

Gaussian distribution of data was confirmed before testing using the DAgostinoPearson normality test

Acknowledgments Our gratitude to Organoid Working Group (OWG) of Centre for Biomedical Research on Rare Diseases (CIBERER), and Institute of Rare Diseases Research (IIER)