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Description
Cancer 106, 14811485

4050 g of plasmid DNA was mixed with 200 l of 2.5 M CaCl 2 and 1.7 ml H 2 O, after which 2 ml of 2xHBS buffer (50 mM HEPES pH 7.05, 140 mM NaCl, 1.5 mM Na 2 HPO 4 2H 2 O) was added dropwise while vortexing or bubbling the solution to enable formation of precipitates

Compared to the rapid blood elimination of free siG-Cy5 and AsiG-Cy5, the nanosystem greatly prolonged the circulation time of siG-Cy5 in T-AsiG-Cy5-CPL, indicating that CPL can effectively protect and deliver siG to the tumor site during circulation

View Abstract Vector insertion, mutagenesis and transgene toxicity