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Subsequently, 50 L of the reaction mixture (4 mM INT, 6.4 mM NAD, 320 mM lithium lactate, 150 mM MPMS in 220 mM Tris-HCl, pH = 8.2) was added to each well and incubated in the dark at room temperature for 30 min
Note: Glutathione is not FDA-approved for medical use
