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Shearer, M

Animal studies have shown differences in affinity to TGR5 based on whether BAs are conjugated with glycine, taurine or are non-conjugated, with Tau-conjugated BAs acting as the most potent agonist 66

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The east to west transit across the Isthmus of Panama was about 30 miles (48 km) by native dugout boats (later modified lifeboats were used) up the often wild and dangerous Chagres River and then by mule back for the final 20 miles (32 km) over the old Spanish trails