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Pellets were grinded with pestles in 100 l of the same buffer, cells were lysed by two freeze-thaw cycles and insoluble material was removed by filtration in a COSTAR R Spin-X centrifuge tube filter
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Female ICR mice were administered Sch A or Sch B [single oral bolus dose: alone (1 mmol/kg) or in combination (0.5 mmol/kg)], as described in Materials and Methods
Similarly, a dietary mixture containing 3% of a clinoptilolite-based product showed an increase in the nitrogen excretion in feces and a decrease in the nitrogen excretion in urine in growing pigs
