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The supernatant was discarded carefully and the cells were resuspended in 3 ml of chilled (80 mM CaCl 2 and 50 mM MnCl 2 ) buffer and kept on ice for 30 min
That's the number you actually use

Studies show that ashwagandha supplementation can reduce stress and anxiety while promoting overall relaxation and mental clarity

Coupling the modules of EMT and stemness: a tunable stemness window model
