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Since then, however, interest has exploded

The % of cytotoxicity was measured using the following formula: Click-iT plus TUNEL assay To perform TUNEL Alexa Fluor Staining, the commercial Click-iT TUNEL Alexa Fluor Imaging Assay (C10247, Invitrogen , Warsaw, Poland) was used in accordance with the instructions provided by the manufacturer

Compared to cyanocobalamin, it is characterised by a stronger binding to tissue and serum proteins, which means a delayed excretion with the urine and thus a longer availability for absorption into the tissue stores