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Methods Molecular Biology Methods Mutagenesis Megaprimer PCR-based mutagenesis [39] was applied to create single-point (W34A) and four-point (R38A/E45A/E49A/R145A) mutations in both ApoE3/4 WT and ApoE3/4 M variants purchased from GeneArt (Thermo Fisher Scientific)

Other symptoms include coughing, decreased appetite, noticeably increased respiratory rate and effort, loss of muscle mass, and abdominal swelling from fluid buildup

This test may be used to help diagnose and monitor various conditions, including: Primary Carnitine Deficiency: An inherited disorder, that reduces carnitine levels, and can cause muscle weakness, heart problems, and metabolic disturbances

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