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Prepare small solvent aliquots to minimize freezethaw cycles and reduce exposure to air/light during handling
Additionally, luteolin enhances lung function by improving epithelial sodium channel (ENaC) expression and reducing lung tissue edema
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For this, 10 l supernatant was transferred to white 96-well plates (Thermo Scientific, 136101) and 50 l PBS containing 5 ng l 1 coelenterazine (CAS 55779-48-1, Santa Cruz Biotechnology, sc-205904) was added to each well
