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A schematic of the regulation of metform concentration in KRAS - wildtype and KRAS -mutation CRC cells Metformin has been reported as a potentially novel antitumor agent in many experiments, but its therapeutic activity is discrepant and controversial so far

Raw data were processed using MS-DIAL software (4.48) 60 to perform peak-picking, alignment, lipid annotation, quantification and polarity amalgamation

Column chromatography was performed on silica gel (Kieselgel 60, 70230 mesh, Merck, Darmstadt, Germany), and semi-preparative HPLC was performed using a Waters HPLC system (Waters, Milford, MA, USA) equipped with two Waters 515 pumps with a 2996 photodiode-array detector

Subsequently, the biotin group in these labeled peptides will be cleaved off by either enzymes (isoTOP-BPP approach) or UV (QTRP and DiaAlk approaches) at a specific wavelength, allowing labeled peptides to be readily analyzed by LC-MS/MS (see Figure 6)