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During this process, as DNA antigenic epitopes are exposed, the simultaneous activation of both TLR9 and cGAS initiates the TLR9MyD88 signaling pathway, resulting in substantial production of IFN [123]

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The following day, the slides were washed extensively and appropriate fluorescently labeled secondary antibodies (Alexa Fluor 488 Goat Anti-Rabbit IgG (H + L), used at 1:500 dilution, Jackson ImmunoResearch Laboratories, 111-545-045) were applied for 2 h at 37 C