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Description
Fifty milliliter of culture was transferred to the anaerobic chamber

Flow cytometry Cultured cells were dissociated with 2 mM Ethylenediamine tetra-acetic acid (EDTA, Sigma) in PBS at 37 C, collected and washed in cold MACs buffer (0.5% bovine serum albumin, BSA, 2 mM EDTA in PBS)

Cope, A

The "cocktail" of vitamins that most of us are deficient had me leaving feeling great
