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The MTT assay was performed according to the instructions, and absorbance was measured at 490 nm

Rather than targeting fat cells directly, it intervenes in the brain's hunger-satiety circuitry, reducing the drive to eat

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It is also worth noting that while GHRP5 was easily soluble in the solution containing SDS micelles, GHRP6, which has a very similar structure and also the UV and ECD spectra in aqueous solutions differed only slightly, immediately formed aggregated precipitates
