in vivo lipid ii assay 14 c Visualizing conformation transitions of the flippase MurJ Biofunctional lipid nanoparticles for precision

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Unilamellar vesicles were obtained by sequential extrusion of the MLVs dispersions in an Avanti Mini-Extruder, through a polycarbonate membrane of 100 nm pore size at around ten degrees centigrade above the transition temperature of the lipid mixture in the appropriate proportion taking into account the literature transition temperature [14-18]

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