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Description
Staining was done using a fixative/staining solution (0.5 % crystal violet, 6 % glutaraldehyde) for 30 min, following extensive rinse with tap water
Eussen SJ, Ueland PM, Clarke R, Blom HJ, Hoefnagels WH, van Staveren WA, et al
"Moulds, yeasts, and aerobic plate counts in supplements." Int J Food Microbiol
Then, ATP depletion leads to the failure of energy-dependent ion pumps (e.g., Na + /K + -ATPase), resulting in ionic imbalances, including the influx of calcium (Ca 2+ ), sodium (Na + ), and chloride (Cl ) ions into the cell, and the efflux of potassium (K + ) [23]
