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TSPCs in the N or NAC group were lysed in lysis buffer (50 mmol/L Tris-HCl, pH 8.0, 1 mmol/L EDTA, 1% Triton X100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 150 mmol/L NaCl) containing proteinase inhibitors (Thermo Fisher Scientific, Rockford, IL, USA)
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The cycling convention developed for those compounds for legitimate pharmacological reasons
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