glutathione sepharose high performance 4b beads ge Cytiva Suitable for batch GST tagged protein purification and scale Purification Using GST HiTrap® 1

Both in vitro and in vivo studies showed that knockdown of either the H or L subunits of ferritin in GBM CSCs markedly reduced cellular growth, as well as their capacity to form tumors in orthotopic models, which supports the role of ferritin as a key player in the proliferation and invasion of CSCs [3]

208 YangJ.WangP.LiS.LiuT.HuX

Although the mechanism of pathogenesis is unclear, it is thought that the clinical phenotypes of SSMD patients involve complete loss of GPX4 enzymatic function

Cell lysates were obtained using TRIzolTM reagent (15596026, Invitrogen, CA, America) and preserved at 80 C for subsequent analysis