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The strips were then soaked for 10 min in the equilibration buffer II containing 50 mM Tris HCl (pH 8), 8 M urea, 30% glycerol, 2% SDS, and 4.5% iodoacetamide
and water
310 A key benefit of this approach is that the information in each chamber is linked to a known genotype and can sometimes be monitored in real-time
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