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After blocking with 5% BSA in TBST, slides were stained with rat anti-BrdU antibody (which detects CldU but not IdU, OBT0030, 1:2000, AbDSerotec) and mouse anti-BrdU antibody (which detects IdU but not CldU, 7580, 1:1000, BD Biosciences) at 4 C for overnight

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The immobility time was defined as the amount of time that rats were suspended passively and remained completely motionless ( Sample Collection and Preparation After the final TST, the rats were decapitated under anesthesia (10 mg/kg of 20% urethane)

Data collection and structural determination The crystal diffraction data of FabX, FabXholo-ACP, FabXoctanoyl-ACP were collected at BL19U1 beamline of National Facility for Protein Science in Shanghai (NFPS) at Shanghai Synchrotron Radiation Facility (SSRF), and processed with HKL3000 or autoPROC 53,54,55 (Table S2)