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Fluorescence imaging for monitoring the colocalization of two single molecules in living cells

These mechanisms represent potential therapeutic targets for CF treatment through modulation of post-translational modifications in the CFTR maturation pathway (Zaman et al., 2019)

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The column oven temperature was initially set at 70 C for 2 min, followed by a gradual increase to 180 C at a rate of 7 C/min, then to 250 C at 5 C/min, and finally to 310 C at 25 C/min, where it was held for 15 min