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Billottet C, Quemener C, Bikfalvi A

Hiltunen et al

pylori, the formed glutamate can be taken up and converted to 2-ketoglutarate by the bacteria and channeled into the TCA cycle 21,29 , an important metabolic pathway that provides precursors for a variety of cell components, as well as for supplying energy to the bacterium 31

Materials Chemicals and reagents Acetonitrile (HPLC-grade): Sigma-Aldrich # 34851 Acivicin: Sigma-Aldrich # A2295 Boric acid : Sigma-Aldrich # B6768 Bovine serum albumin: Sigma-Aldrich #A3294 Bradford reagent: Supelco #B6916 Brij L23 solution: Sigma-Aldrich # B4184 Disodium hydrogen phosphate: Sigma-Aldrich # 30412 Dithiothreitol: Millipore # 111474 Drabkins reagent : Sigma-Aldrich, # D 5941 Glacial acetic acid (HPLC-grade): EMD Millipore Chemicals # AX0074 l -Glutathione, reduced: Sigma-Aldrich # G4251 HPLC Zorbax Eclipse XDB-C18 column 4.6 150 mm, 5 m: Agilent Technologies Human hemoglobin : Sigma-Aldrich # H7379 Hydrochloric acid 37%: Sigma-Aldrich # 320331 Methanol (HPLC-grade): Sigma-Aldrich # 34860 Monobromobimane : Millipore # 596105 N -ethylmaleimide : Sigma-Aldrich # E1271 Phosphate buffer solution 1.0 M pH 7.4: Sigma-Aldrich # P3619 Potassium dihydrogen phosphate: Sigma-Aldrich, # 229806 l-Serine: Sigma-Aldrich # S4500 Sodium chloride: Sigma-Aldrich # S7653 Sodium hydroxide solution, 2 M: Fluka # 35254 Trichloroacetic acid: Sigma-Aldrich # T6399 Tripotassium EDTA: Fluka # 03664 Tris base: Sigma-Aldrich # T1503 Water (HPLC-grade): Sigma-Aldrich # 34877 Equipment The chromatographic separations reported in this article were performed using an HPLC Agilent 1100 series with fluorometric detector (Agilent Technologies)