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3b), despite neurons expressed mito GCL, indicating that GPx1 use of -glutamylcysteine is essential for -glutamylcysteine-mediated O 2 detoxification in this model of excitotoxicity

Collected cells were resuspended into Cellartis DEF-CS medium supplemented with GF-1, GF-2, and GF-3 and seeded onto a 6-well tissue culture plate pre-coated with LDEV-free, hESC-qualified Matrigel (Corning Inc.) at a cell density of 2.1x105 cells/cm2

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