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Two important ALDH isoenzymes involved in this process are ALDH1 and ALDH2 ( In addition to ALDH, DSF is also known to inhibit other enzymes involved in vital metabolic pathways such as glycolysis, tricarboxylic acid cycle, pentose phosphate shunt, glutathione system, and catecholamine synthesis through S-S linkage or by chelation of copper or zinc from the active sites of the enzymes ( Anticancer Mechanisms of Disulfiram The anticancer activity of DSF has been demonstrated in various cancer cells models and is shown to be strongly dependent on formation of Cu(DDC)2 complex with divalent metal ions such as Cu