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Human tumor samples Clinical samples, including 12 pairs of primary GC, adjacent normal gastric, and peritoneal metastasis samples for bulk RNA sequencing, 172 primary GC tumor samples, with 97 paired adjacent normal gastric tissue samples, and 16 paired peritoneal metastasis samples for IHC analysis, and GC primary tumor samples for the establishment of orthotopic GC models and GC organoids were collected from Sun Yat-sen University Cancer Center (Guangzhou, China)

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Fluorescence spectra were acquired using an FS5 spectrofluorometer (Edinburgh Instruments) equipped with an SC-40 plate reader and 96-well plates (Corning) using Fluoracle software